Figure 12.

Fluorescence and the corresponding phase images of untreated B16 cells and cells treated with Olax, b-AgNPs c-AgNPs, observed by an Olympus Fluorescence Microscope. Fluoresence images of B16 cells treated with (a) untreated or control, (b) Olax (100 µg/ ml) leaf extract ,(c) b-AgNPs (at 30 µM) and (d) c-AgNPs (at 30 µM). Images of a', b', c' & d' correspond to phase images. All the treated B16 cells were extensively washed with DPBS (6 times) before taking the fluorescence images. It is to be noted that there is no significant cell killing is observed at 30 µM.