Table 1. List of primary antibodies.
| |
|
|
|
Dilution |
||
|---|---|---|---|---|---|---|
| Antigen | Product name and supplier | Host species and clonality | Buffer | IHC | IF | Fetal IF |
| TET1 |
TET1 (Y-14), sc-163446, Santa Cruz Biotechnologies, Inc. |
Goat polyclonal |
TEG |
1 : 100 |
1 : 100 |
− |
| TET2 |
TET2 (S-13), sc-136926, Santa Cruz Biotechnologies, Inc. |
Rabbit polyclonal |
CIT |
1 : 2000 |
1 : 1500 |
− |
| APOBEC1 |
APOBEC1 anti-Human (N-Terminus) Antibody, LS-C98890, LifeSpan BioSciences, Inc., Seattle, WA, USA |
Rabbit polyclonal |
TEG |
1 : 100 |
1 : 150 |
1 : 50 |
| MBD4 |
MBD4 antibody, NBP1-91189, Novus Biologicals, Cambridge, UK |
Rabbit polyclonal |
CIT |
1 : 1000 |
1 : 800 |
1 : 200 |
| APEX1 |
APEX1 / APE1 Goat anti-Human Polyclonal (N-Terminus) Antibody, LS-B3924, LifeSpan BioSciences, Inc. |
Goat polyclonal |
CIT |
1 : 100 |
1 : 100 |
1 : 50 |
| PARP1 |
Anti-PARP antibody, ab6079, Abcam, Cambridge, UK |
Rabbit polyclonal |
CIT |
1 : 150 |
1 : 150 |
1 : 75 |
| XRCC1 |
Anti-XRCC1 antibody, ab1838, Abcam |
Mouse monoclonal |
CIT |
1 : 50 |
1 : 50 |
− |
| 5mC |
Anti-5-Methylcytosine Mouse mAb (162 33 D3), NA81, Calbiochem, Damstadt, Germany |
Mouse monoclonal |
-MBO |
1 : 200 |
− |
− |
| 5hmC |
5-hmC monoclonal antibody (Mab-31HMC), Diagenode, Liege, Belgium |
Mouse monoclonal |
-MBO |
1 : 1000 |
− |
− |
| DNMT1 |
DNMT1 Rabbit anti-Human Polyclonal (aa1603-1616) Antibody, LS-B800, LifeSpan BioSciences, Inc. |
Rabbit polyclonal |
CIT |
1 : 3000 |
1 : 3000 |
− |
| DNMT3A |
Dnmt3a (H-295), sc-20703, Santa Cruz Biotechnologies, Inc. |
Rabbit polyclonal |
CIT |
1 : 600 |
1 : 600 |
− |
| DNMT3B |
Dnmt3b (Q-25), sc-130740, Santa Cruz Biotechnologies, Inc. |
Rabbit polyclonal |
CIT |
1 : 75 |
1 : 50 |
− |
| DNMT3L |
A kind gift from Dr. Okamoto, Tokyo, Japan |
Rabbit polyclonal |
TEG |
1 : 5000 |
1 : 5000 |
− |
|
CIS and fetal germ cell markers | ||||||
| D2-40 (CIS) | Anti-Human D2-40, M3619, Dako | Mouse monoclonal | CIT | 1 : 100 | 1 : 100 | − |
| PLAP (CIS) | Anti-Human Placental Alkaline Phosphatase, M7191, Dako | Mouse monoclonal | TEG | − | 1 : 100 | − |
| MAGE-A4 (fetal GC) | Kind gift from Dr. Guilio Spagoli, Basel, Switzerland | Mouse monoclonal | TEG+CIT | − | 1 : 250 | |
| OCT 3/4 (fetal GC) | Oct-3/4 Antibody (C-10), sc-5279, Santa Cruz Biotechnologies, Inc. | Mouse monoclonal | CIT | − | − | 1 : 50 |
Abbreviations: CIS=carcinoma in situ; CIT=citrate buffer (10 mM, pH 6); GC=germ cells; IF=Immunofluorescence; IHC=immunohistochemistry; -MBO=no buffer and microwave treatment; TEG=TEG buffer (10 mM Tris, 0.5 mM EDTA, pH 9).
Antibodies were used at indicated dilutions and buffers.