Table 2. Staining intensities of potential demethylating proteins and DNA methylases observed in CIS by immunohistochemistry.
| Antigen | Nucleus | Cytoplasm |
|---|---|---|
| TET1 N=6 |
neg, few + |
neg, few + |
| TET2 N=6 |
neg, few + |
neg, few + |
| APOBEC1 N=8 |
neg, + |
+++ |
| MBD4 N=8 |
+, ++, few neg |
neg |
| APEX1 N=8 |
++, +++, few neg |
neg |
| PARP1 N=8 |
+, ++, few neg, few +++ |
neg |
| DNMT1 N=8 |
+, ++, few neg |
neg |
| DNMT3A N=8 |
neg |
neg |
| DNMT3B N=8 |
neg, few + |
neg |
| DNMT3L N=8 | neg and ++ | neg |
For each tissue sample, the intensity of the staining of CIS cells, was evaluated and scored as negative (neg), faint staining (+), moderate staining (++) and strong staining (+++). For each antigen, the general staining pattern observed across the investigated tissue samples was subsequently summarised, by collectively describing all the staining intensities observed in the included samples (N). If a staining intensity was observed only in a few (1–2) of the included tissue samples, this was noted (few).