Figure 5.

The fate of the vessel sprout. (A) A creation of new vessel connection. The sprout (arrows) connected with an existing capillary after 8 days (8d) of hypoxia (center). After 11 days (11d) of hypoxia, the shape of this newly formed vessel becomes normalized, similar to the other existing capillaries (right). Note that a lumen of the new vessel was completely sealed with green fluorescent protein (GFP)-expressing endothelial cells. (B) An elimination of the sprout. The sprout (arrows) observed after 8 days of hypoxia (center) was eliminated after 11 days of hypoxia (right). For visual purposes, the images are shown as the maximum intensity projection.