Figure 5.

Endothelial progenitor cells (EPC)-conditioned media does not rescue axon degeneration after stretch injury. (A) Cortical neurons labeled for α-tubulin in control and stretch conditions. Arrowheads indicate α-tubulin breakdown and beading indicative of axonal degeneration. (B) Stretch injury resulted in a significant increase in axonal degeneration relative to control cultures (*P=0.018). Treatment with EPC-conditioned media did not result in axonal protection; n=5 independent stretch experiments performed in triplicate. (Scale bar, 50 μm).