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. 2013 Dec 4;34(2):357–366. doi: 10.1038/jcbfm.2013.216

Figure 6.

Figure 6

Endothelial progenitor cells (EPC)-conditioned media rescues oxygen–glucose deprivation (OGD)-induced axon degeneration. Cortical neurons labeled for α-tubulin in panel A control, (B) OGD, (C) OGD+EPC-treated and (D) OGD+EPC-conditioned media-treated cultures. Arrowheads indicate α-tubulin breakdown. (E) OGD did not adversely affect the total number of live neurons in culture. However, coculturing with EPCs resulted in a significant reduction in the number of dead cells present in the culture (**P<0.001) relative to other treatment groups. (F) Sublethal OGD exposure resulted in significant axonal degeneration (*P=0.01) relative to normoxia controls. Coculturing of EPCs with OGD-injured neurons did not result in axonal protection. Addition of EPC-conditioned media to OGD cultures resulted in a significant reduction in axonal degeneration relative to OGD cultures (#P<0.01). (G) Coculturing of OGD neurons with fibroblasts or fibroblast-conditioned media did not result in axonal rescue; n=5 independent OGD experiments performed in triplicate.