Table 3.
Enzyme | Gene | Expression system | Referencesa |
---|---|---|---|
SMO | styA1/StyA2B from R. opacus 1CP | E. coli BL21(DE3) pLysS/pET16bP | Tischler et al., 2009, 2010 |
wild-type and mutant styAB fragment from P. putida CA-3 | E. coli BL21(DE3)/pRSET-B | Gursky et al., 2010; Boyd et al., 2012; Nikodinovic-Runic et al., 2013 | |
PAMO | different mutant pamO from T. fusca | E. coli TOP10/pBAD/myc-HisA | Dudek et al., 2011 |
wild-type and mutant pamO from T. fusca | E. coli TOP10/pBAD-Tat-AldO plasmid (periplasmic expression) | Dudek et al., 2013a, b | |
BVMO | 9 bvmo genes from R. jostii RHA1 | E. coli TOP10/pBADN and pCRE2 (phosphite dehydrogenase gene) | Riebel et al., 2012 |
bvmo gene from A. radioresistens S13 | E. coli BL21(DE3)/pT7 expression vector | Minerdi et al., 2012 | |
FMO (type I) | fmo from Methylophaga sp. SK1 | E. coli TOP10/pCRE2 (soluble NADPH regenerating phosphite dehydrogenase) | o, Gotor and Fraaije, Rioz-Martínez et al. |
tmm gene from M. silvestris | E. coli BLR(DE3) pLysS/pET28a | Chen et al., 2011 | |
FMO (type II) | fmo gene from S. maltophilia | E. coli BL21(DE3)/pET-YSBL-LIC-3C | Jensen et al., 2012 |
several fmo genes from R. jostii RHA1 | E. coli/pBADN and pCRE2 | Riebel et al., 2013a | |
CHMO | chmo gene from A. calcoaceticus NCIMB 9871 and mutant fdh (formate dehydrogenase) gene from C. boidinii | E. coli BL21(DE3)/pET22b (chmo) and pACYCDuet-1 (fdh or both genes in tandem) | Zhai et al., 2013 |
The information shown mainly corresponds to reports as of 2010.
Abbreviations: BVMO, Baeyer-Villiger monooxygenase; FMO, flavin-containing monooxygenase; CHMO, cyclohexanone monooxygenase; fdh, formate dehydrogenase; HAPMO, 4-hydroxyacetophenone monooxygenase; PAMO, phenylacetone monooxygenase; SMO, styrene monooxygenase; tmm, trimethylamine monooxygenase.