Figure 3. Photobiological H₂ production in DCMU-treated cells under anaerobic conditions with continuous illumination.

The tested cells were equally divided into 60 mL glass tubes and then sealed with rubber stoppers after being sparged with argon gas. All of the samples were incubated in a 30°C culture box with 100 μE·m⁻²·s⁻¹ illumination. The H₂ content in the untreated samples and the DCMU-treated samples was then measured by a GC system at specific time points. Compared to the untreated cells (red symbol and line), H₂ accumulation in the DCMU-treated samples increased quickly and lasted more than 120 hours. The 0 hour, 3 hour, 6 hour, 12 hour and 24 hour time points were recorded as the early phase, and after the 24 hour point, the H₂ accumulation was recorded every 24 hours. Error bars indicate s.d. values calculated from the average of triplicate experiments.