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K394R-β-catenin ubiquitination is unaffected by Rad6B silencing. MDA-MB-231 cells were transfected with nontarget (NT) or Rad6B (R6B) shRNA and myc-tagged wild type (WT), K354R- or K394R-β-catenin and HA-ubiquitin. (A) Western blot analysis. (B and C) Graphic representation of Rad6 and ubiquitinated β-catenin, respectively. * and ns indicate significant (P<0.001) and no significant difference, respectively.
