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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Muscle Res Cell Motil. 2013 Oct 6;34(0):395–405. doi: 10.1007/s10974-013-9362-9

Figure 5. Line scanning of laminin and α-DG immunoreactivity.

Figure 5

Immunofluorescent colocalization of laminin and α-DG was measured by confocal microscopy. Line scans of membrane interfaces expressing both laminin and α-DG were collected and analyzed. (a) Representative images demonstrating example line scans. (b) Representative normalized line scans from WT, mdx, and dko sections. The dashed line represents 50%, used to calculate the distribution of immunofluorescence. (c) Summary of the distribution of immunoreactivity for laminin and α-DG. n= 419–596 individual line scans. * P<0.0001 versus WT α-DG distribution, † P< 0.0001 versus WT laminin distribution