Fig. 6.

FOXO1 regulates expression of endogenous AR target genes in 22Rv1 CRPC cells. A: Schematic diagram of AR mRNA and protein, AR siRNA targeting sites and major AR splice variants identified in 22 Rv1 cells. B,C: 22Rv1 cells were transfected with non-specific (NS) or AR siRNAs and empty vector or FLAG-tagged FOXO1. At 48 hr after transfection, cells were harvested for Western blot analysis (B) or real-time RT-PCR (C). Immunoblotting of extracellular signal-regulated kinase 2 (ERK2) was included as a loading control. Columns, mean among three individual experiments; bars, SD.