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. 2013 Sep 1;140(17):3552–3564. doi: 10.1242/dev.096099

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Abdominal-B suppresses the formation of neuroblasts in parasegment 15. (A-F) Flat preparations (maximum projections) of St12e and St13 embryos of the indicated genotype double-stained as illustrated. PSs are depicted on the right and their borders are illustrated by a solid line. (A-C) Dpn serves as a universal NB marker. (D-F) NB2-4 (dorsal location) and NB3-3 clones (ventral location) are En negative and indistinguishable in maximum projections of PS13 (yellow asterisks). The typical medial location of the NB7-3 cluster (En positive) is marked by yellow arrowheads. (D,F) The abdominal NB6-4 clones (consisting of two glia) already downregulated Eg expression. (E) Transformation of abdominal NB6-4 into thoracic fate is indicated by appearance of the neuronal subclone, showing constant Eg expression (white arrows). Ectopic NB7-3 cells in PS15, which are not found in wild type or in Df(3L)H99 mutant embryos, are surrounded by broken lines and indicated by white arrowheads. (G-J′) The ectopic NB7-3 cells in PS15 of Abd-B^D18 mutant embryos were identified by double-staining against Eg (green) and various molecular markers as indicated (red). (K) Statistics for the total number of Dpn-positive NBs (per side) of the indicated genotypes in PS13, PS14 and PS15, respectively. ^*P<0.05; ^***P<0.001. Error bars represent s.d. ML, midline; WT, wild type.