Fig. 5.

FasIII is a homophilic adhesion molecule. (A-A′) Clonal boundary within a third instar wing imaginal disc containing regions lacking FasIII (FasIII^-/-) and wild-type regions (FasIII^+/+). FasIII is preferentially localised on intercellular boundaries shared by wild-type cells (arrowheads). (B-B′) Exogenously expressed FasIII and endogenous F-Actin in Kc₁₆₇ tissue culture cells. FasIII is localised on plasma membranes abutting other FasIII-expressing cells (arrowheads). Note that 100% of FasIII expressing cells are seen in clumps, and non-expressing cells are never seen in clumps. (C,D) Third instar wing imaginal discs containing GFP labelled, but otherwise wild-type (C) or FasIII^A142 mutant (D) clones. (E) Quantification of average clonal boundary curvature using a 15-μm smoothing window (as described in Materials and methods). Box represents 25th to 75th percentile; line represents the median; whiskers were calculated using the Tukey method with outliers shown. P-value was determined by a two-tailed Mann-Whitney test.