Post-embryonic Ilp7 neurons selectively innervate reproductive tracts. Using stochastic GAL80 Flp-out to generate mosaic Ilp7-GAL4,UAS-mCD8::GFP expression, we imaged individual neurons from each Ilp7-subset. (A,C,E) Cartoons depict the mosaically labeled neurons in the images below. Green circles indicate the neurons in which GAL80 was excised for the images shown; gray circles indicate all other Ilp7 neurons. (B,D,E) Images of labeled neurons within ventral (Ven) and dorsal (Dor) subsets. Fkh is expressed in embryonic Ilp7 neurons (arrows), but not post-embryonic Ilp7 neurons (arrowheads). (B-B′) The large embryonic Ilp7 neurons innervate the gut only. (D-D′) Female-specific post-embryonic Ilp7 neurons innervate the oviduct only. (F-F′) Post-embryonic Ilp7 neurons of the dorsal cluster innervate the oviduct only in females. Arrows/arrowheads indicate representative neurons of each Ilp7 subset.