Table 1.
Nucleotide sequences of oligonucleotides designed for assembly of IFN-CSPa,b.
| Primers | Nucleotide sequences (from 5′ end to 3′ end) |
|---|---|
| IC-1 | GGAATTCCATATG TGTGATCTGCCTCAAA |
| IC-2 | GTGCCAGGAGCATCAAGGTCCTCCTGCTACCCAGGCTGTGGGTTTGAGGCAGATCACA |
| IC-3 | ACCTTGATGCTCCTGGCACAGATGAGGAGAATCTCTCTTTTCTCCTGCTTGAAGGACA |
| IC-4 | GGTTGCCAAACTCCTCCTGGGGAAATCCAAAGTCATGTCTGTCCTTCAAGCAGGAGAA |
| IC-5 | CAGGAGGAGTTTGGCAACCAGTTCCAAAAGGCTGAAACCATCCCTGTCCTCCATGAGA |
| IC-6 | AGTCCTTTGTGCTGAAGAGATTGAAGATCTGCTGGATCATCTCATGGAGGACAGGGAT |
| IC-7 | CTCTTCAGCACAAAGGACTCATCTGCTGCTTGGGATGAGACCCTCCTAGACAAATTCT |
| IC-8 | AGGCTTCCAGGTCATTCAGCTGCTGGTAGAGTTCAGTGTAGAATTTGTCTAGGAGGGT |
| IC-9 | CTGAATGACCTGGAAGCCTGTGTGATACAGGGGGTGGGGGTGACAGAGACTCCCCTGA |
| IC-10 | GGAAGTATTTCCTCACAGCCAGAATGGAGTCCTCCTTCATCAGGGGAGTCTCTGTCAC |
| IC-11 | GCTGTGAGGAAATACTTCCAAAGAATCACTCTCTATCTGAAAGAGAAGAAATACAGCC |
| IC-12 | ATCTCATGATTTCTGCTCTGACAACCTCCCAGGCACAAGGGCTGTATTTCTTCTCTTT |
| IC-13 | AGAGCAGAAATCATGAGATCTTTTTCTTTGTCAACAAACTTGCAAGAAAGTTTAAGAA |
| IC-14 | CTTAATTTCTCGTTGTCTTCCTTACTTCTTAAACTTTCTTGCAA |
| IC-15 | AAGACAACGAGAAATTAAGGAAACCAAAACATAAAAAATTAAAGCAACCAGCGGA |
| IC-16 | CCGCTCGAGACCATCCGCTGGTTGCTTTAA |
aRestriction sites are bold.
bThe italicized letters indicate the overlapped parts in IFN-CSP.