Labeled MCA-RH7777 cells and unlabeled BRL-3A cells grown and transfected in co-culture showed high transgene expression in hepatoma cells with much lower levels of transfection in hepatocytes. Brightfield images are uncolored, eGFP signal is green, and H2B-cherry signal is red. Each set of four images shows brightfield merged with eGFP and H2B-cherry (top left), eGFP and H2B-cherry (top right), eGFP only (bottom left), and H2B-cherry only (bottom right).