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(A) H2B-cherry signal of hepatoma cells allowed separate gating of the two populations with flow cytometry. The top population (tinted pink) shows H2B-cherry+ hepatoma cells; the bottom (tinted yellow) is unlabeled hepatocytes. Transfection efficacy measured by (B) percent of cells transfected and (C, D) geometric mean fluorescence intensity showed high specificity for MCA-RH7777 cells over BRL-3A cells in co-culture studies.
