Fig. 4.

Activated microglia (OX42) immunohistochemistry of brain slices of the frontal and parietal cortices and thalamic region after CIP. OX42 immunoreactivity was strongly expressed at 3 and 48 h. Activated microglial cells were noted by their stout, dense appearance with stellate projections. At 72 h, OX42 immunoreactivity was decreased compared with 3 and 48 h, with immunoreactivity primarily in the parietal cortex and to a lesser extent in the frontal cortex (n = 3/time point). Positive (vimentin immunoreactive) and negative (anti-IgG) controls were routinely performed to ensure consistency.