Longitudinal measurements of RNFLT and RT obtained by SD-OCT are shown for a single representative rat at baseline (A, B) and at 1 week (C, D) and 2 weeks (E, F) after acute, unilateral IOP elevation to 50 mm Hg for 8 hours. Data from the experimental eye (Exp) appear in the left column and from the fellow control eye (Ctrl) in the right column. In each panel, the SD-OCT B-scan is shown alongside the infrared ocular fundus CSLO image. The B-scan position is indicated by the red circle in the CSLO image and is maintained at follow-up with eye tracking software. B-scan images show the segmentations of the inner limiting membrane (green), outer border of the nerve fiber layer (blue), and Bruch's membrane–RPE complex (yellow), used to calculate peripapillary RNFLT (green to blue) and RT (green to yellow). The RNFLT increased at the 1-week and 2-week follow-up (C, E) compared with baseline (A) for the experimental eye, while the fellow control eye remains consistent (B, D, F). Scale bars apply to (A) through (F).