Figure 4.

Results of the retrograde axonal transport assay 2 weeks after the 8-hour episode of acute IOP elevation to 50 mm Hg in the experimental eye in one representative animal. (A, B) In vivo CSLO-FL images of the experimental (A) and fellow control (B) ocular fundi showing equivalent fluorescence (intensity and density) of RGC somas 24 hours after bilateral injections of CTB into the superior colliculi. (C, D) Postmortem fluorescence microscopy (MS-FL) of experimental (C) and fellow control (D) retinal whole mounts also shows equivalent fluorescence between eyes. The fluorescence of the RGCs is similar between the CSLO and microscopy images, although the optic disc is usually brighter in the microscopy image. Note that the disc in (D) has been partially removed during the retina dissection, so it is not as bright as the disc in C. These results indicate uninterrupted retrograde axonal transport of CTB along experimental and control pathways. Quantitative results are summarized in Table 3. (E) Postmortem CSLO-FL image of the superior colliculi shows that CTB injections were successful. Scale bars: 1 mm (A applies to A–D).