Figure 1. N-MuSK 60 Purification.
The culture media from COS7 cells transfected with N-MuSK 60 vector (M60) or empty vector (V) was applied to a Ni column and eluted with buffer containing imidazole. A. Western blot demonstrating a strong immuno-reacting band in original culture medium and first (E1) and second (E2) effluent fractions. B. Coomassie stained SDS-gel electrophoresis. The immuno-reacting band in the second elution fraction corresponds to a single Coomassie-stained band.