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Sera diluted from 1:104 through 1:106 from rat immunized with 100ug N-MuSK 60 (titer >1:106) and one immunized with 50ug (titer=1:105) blotted against 0.5ug affinity purified mouse N-MuSK 60 or 0.5ug BSA as an antigen control. Serum from three adjuvant control animals diluted 1:500 (lower panel) showed no reaction.
