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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Allergy. 2013 Nov 23;68(12):1546–1554. doi: 10.1111/all.12261

Figure 1. The binding of rAra h 1 to RAGE before and after the Maillard reaction in the presence of glucose or xylose.

Figure 1

Panel A is a Western blot with anti-Ara h 1 antibody binding to 1) untreated rAra h 1 control, 2) untreated rAra h 1 supernatant from pull-down with RAGE-bound amylose resin or 3) amylose resin alone and 4) co-elution with MBP-RAGE from amylose resin or 5) amylose resin alone. Panel B is a anti-Ara h 1 Western blot of 1) untreated rAra h 1, 2) heated with glucose or 3) xylose for 10 days, 4) xylose treated rAra h 1 supernatant from pull-down with RAGE, or 5) co-elution with MBP-RAGE from amylose resin , and 6) glucose treated rAra h 1 supernatant from pull-down with RAGE, or 7) co-elution with MBP-RAGE from amylose resin , 8) Molecular weight marker.