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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Allergy. 2013 Nov 23;68(12):1546–1554. doi: 10.1111/all.12261

Figure 4. Detecting the binding of Ara h 1, Ara h 2 and Ara h 3 from raw and roasted peanut extracts to RAGE.

Figure 4

SDS-PAGE (Panel A) and Western blot of pull down experiments with (Panel B) anti-Ara h 1 , (Panel C) anti-Ara h 2, (Panel D) anti-Ara h 3 antibodies, and (Panel E) anti-IgE from pooled sera of patients #2 and #3 are shown. The lanes are: 1) Raw and 2) roasted peanut extracts that were subjected to MBP-RAGE amylose resin pull down experiments. Lanes 3 and 5 of each panel are supernatants from pull down experiments and lanes 4 and 6 contain material from raw and roasted peanut extracts, respectively, that co-elute with the RAGE from amylose resin. MW indicates the molecular weight marker. All panels are scaled to the molecular weight markers at the left of panel B.