Table 1.
AGE Modifications on rAra h 1
| Recombinant Ara h 1 + xylose | |||||
|---|---|---|---|---|---|
| Peptide Sequence | m/z | MW obs (M+H) | MW theor (M+H) | delta MW | Modification |
| GPLLSILKAFN | 616.2 | 1231.4 | 1230.7 | 0.7 | CML |
| IFLAGDKDNVIDQIEK | 626.3 | 1876.9 | 1875 | 1.9 | CML |
| VAKISMPVNTPGQFEDFFPASSR | 862.1 | 2584.3 | 2583.3 | 1 | CML |
| DLAFPGSGEQVEKLIK | 614 | 1840 | 1839 | 1 | Pyrraline |
| IFLAGDKDNVIDQIEK | 643.3 | 1927.9 | 1926 | 1.9 | Pyrraline |
| NTLEAAFNAEFNEI(RR) | 670.9 | 2010.7 | 2009.9 | 0.8 | Di-CML1 |
| Recombinant Ara h 1 + glucose | |||||
| AMVIVVVNKGTGNLELVAVR | 749.2 | 2245.6 | 2244.3 | 1.3 | Fructose Lysine |
1
extensive y-ion series and precursor mass confirm this peptide with the addition of 116 Da, but the position of the 116 Da addition is ambiguous