Table 2.
AGE Modifications in Peanut Extract
| Ara h 1 (raw) | |||||
|---|---|---|---|---|---|
| Peptide Sequence | m/z | MW obs (M+H) | MW theor (M+H) | delta MW | Modification |
| EGALML(hydroxylP)(HFNSK) | 473.2 | 1417.6 | 1417.7 | 0.1 | CM1 |
| Ara h 3 (raw) | |||||
| NGIEETICSASVKK | 769.9 | 1538.8 | 1536.8 | 2 | CML |
| QLKNNNPFKFFVP(hydroxlP)FDHQSMR | 889.7 | 2667.1 | 2666.3 | 0.8 | CM |
| Ara h 1 (roasted) | |||||
| IFLAGDKDNVIDQIEKQAK | 754.6 | 2261.8 | 2261.2 | 0.6 | CML |
| VAKISMPVNTPGQFEDFFPASSR | 862.2 | 2584.6 | 2583.3 | 1.3 | CML |
| SSENNEGVIVKVSK | 517.2 | 1549.6 | 1547.8 | 1.8 | CML |
| DLAFPGSGEQVEKLIK | 597.1 | 1791.1 | 1788.9 | 1.2 | CML |
| Ara h 3 (roasted) | |||||
| NGIEETICSASVKK | 769.9 | 1538.8 | 1536.8 | 2 | CML |
| QLKNNNPFKFFVPPFDHQSMR | 889.7 | 2667.1 | 2666.3 | 0.8 | CM2 |
1
extensive y-ion series and precursor mass confirm this peptide with the addition of 58 Da ut the exact position of the 58 Da addition can only be localized to the 5 C-terminal residues
2
extensive y-ion series and precursor mass confirm this peptide with the addition of 58 Da ocalized on the extreme N-terminal residue