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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Immunol. 2013 Oct 7;191(10):5026–5035. doi: 10.4049/jimmunol.1300013

Figure 5. Impaired humoral immunity following OX40 stimulation during LCMV-Armstrong despite absence of enhanced immunopathology.

Figure 5

(A – G) C57BL/6 mice were infected with LCMV Armstrong and treated with 100 μg rat IgG1 or α-OX40 one day p.i. Mice were euthanized 20 days post infection. CD4 and CD8 T cell numbers were determined by flow cytometry (A and B). (C and D) Intracellular cytokine staining (ICCS) was performed following in vitro stimulation of splenocytes with the GP33 peptide (CD8 T cells) or GP61 peptide (CD4 T cells). FACS-plots are gated on CD8+CD19− cells (C) and CD4 T cells (D). (E) GC B cells were analyzed in IgG and α-OX40 treated mice, FACS-plot are gated on CD19 B cells. (F) Anti-LCMV IgG responses were analyzed by Elisa. (G) Serum samples of treated and untreated mice were harvested on day 20 post infection and viral loads were determined by plaque assay. Data are derived from at least 2 independent experiments with 4-6 mice per group. ***p <0.001.