Figure 5. CD147 expressing primary MM cells are transiting the cell cycle.

A) BM MNCs from two MM patients were cultured overnight at 37°C in the presence of BrdU. MM Patient 1 displayed a bimodal CD147 expression and the gated CD147^bright subset (R2) was shown to be significantly enriched for cells in S and G2/M phases. MM patient 2, which exhibited more uniform CD147 expression, was analyzed by gating CD147 bright and dim populations. The CD147^bright cells (R2) were greatly enriched for cells in S and G2/M. B) Sorted CD147^bright and CD147^dim populations from MM patient 2 were cultured for 5 days before assessing IL-6 and CypB responsiveness as measured by DNA synthesis. C) Forward and size scatter analysis of primary MM patient CD138+/CD147^dim vs. CD138+/CD147^bright cells.