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. 2013 Dec 3;24(2):161–176. doi: 10.1038/cr.2013.155

Figure 5.

Figure 5

Increased activation of the Btk and non-canonical NF-κB pathways is responsible for the increased IFN-γ production in CD40-triggered CD11ahiFcγRIIIhi B cells. (A, B) Signaling pathways in the CD11ahiFcγRIIIhi and conventional B cells stimulated with activating anti-CD40, with actin (A) and lamin A (B) as loading controls. The data are representative of three independent experiments with similar results. The numbers below the lanes (top) indicate Btk (A) and p52 (B, C) band densities, presented relative to the β-actin (A) and lamin A (B, C) expression in the same lane (below). (C) Nuclear translocation of p65 and p52 in CD11ahiFcγRIIIhi B cells pretreated with the Btk inhibitor PCI-32765 (5 nM) for 60 min. (D) Intracellular IFN-γ expression in CD11ahiFcγRIIIhi B cells pretreated with neutralizing CD11a mAbs or the Btk inhibitor PCI-32765.