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. 2013 Nov 19;24(2):233–246. doi: 10.1038/cr.2013.152

Figure 1.

Figure 1

Pat1C interacts with Lsm1-7 complex through Lsm2 and Lsm3 bridging. (A) Domain organization of yeast Pat1. (B) Purified S. cerevisiae proteins, including Lsm1, Lsm2-3 subcomplex, Lsm4N, Lsm5-6-7 subcomplex, Pat1C, and the reconstituted Lsm1-7, Lsm2-3-Pat1C and Lsm1-7-pat1C complexes, are visualized by NuPAGE gradient gel. The positions of each protein are shown by arrows. (C) Native PAGE was used to detect the interaction of individual Lsm proteins or subcomplexes with Pat1C.