Table 1. Libraries of ELP diblock copolymers characterized.
ELP diblock libraries [Xaa:Yaa] | ELP Nomenclature | *Amino acid sequence | **Calculated ELP MW (Da) | ***Observed ELP MW (Da) |
---|---|---|---|---|
Ile:Ser | I18S18 | G(VPGIG)18(VPGSG)18Y | 15,015 | 14,599 |
I24S24 | G(VPGIG)24(VPGSG)24Y | 20,012 | 19,820 | |
I36S36 | G(VPGIG)36(VPGSG)36Y | 29,572 | 29,826 | |
I48S48 | G(VPGIG)48(VPGSG)48Y | 39,643 | 39,749 | |
I96S96 | G(VPGIG)96(VPGSG)96Y | 79,049 | 79,216 | |
Ser:Ile | S48I48 | G(VPGSG)48(VPGIG)48Y | 39,643 | 39,670 |
Ile:Ala | I48A48 | G(VPGIG)48(VPGAG)48Y | 38,946 | 38,940 |
I96A96 | G(VPGIG)96(VPGAG)96Y | 77,655 | 77,233 | |
Ala:Ile | A48I48 | G(VPGAG)48(VPGIG)48Y | 38,946 | 38,952 |
A96I96 | G(VPGAG)96(VPGIG)96Y | 77,655 | 77,840 |
Gene sequence confirmed by N and C terminal DNA sequencing and diagnostic digestion.
Estimated from open reading frame excluding methionine start codon
Results from matrix assisted laser desorption ion time of flight mass spectrometry