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. 2013 Jan 14;1(3):493–498. doi: 10.3892/mco.2013.70

Table II.

Primer sequences, PCR and DHPLC conditions for the detection of gene polymorphisms.

Gene Primer sequence PCR annealing temperature (°C) PCR product size (bp) DHPLC application type Oven temperature (°C)
PSCA F: AGTCACCTGAGGCCCTCTC
R: CTGCAGCCTTTGCTGATGACG
59 241 Mutation 59

PCR, polymerase chain reaction; DHPLC, denaturing high-performance liquid chromatography.