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. 2013 Oct 15;22(2):451–463. doi: 10.1038/mt.2013.201

Figure 1.

Figure 1

Short-term expansion of primary erythroblasts derived from unfractionated human cord blood mononuclear cells (CB MNCs) in a serum-free culture condition. (a) Growth curves of erythroblast expansion cultures started with unfractionated CB MNCs in serum-free medium supplemented with SCF, IL-3, IGF-1, EPO, and Dexamethasone. Two curves represent two different CB donors. (b) Morphology of day 19 primary erythroblast expansion culture from CB MNCs after Wright-Giemsa and Benzidine staining. Majority of the cells have pro-erythroblast or basophilic-erythroblast morphology and are benzidine negative. Scale bar: 10 µm. (c) FACS analysis of cell surface markers and intracellular hemoglobin expression phenotypes of day 19 primary erythroblast expansion culture from CB MNCs. (d) Colony-forming unit (CFU) types and numbers of 10,000 primary culture cells at day 19 erythroblast expansion culture from CB MNCs. No myeloid CFUs and few burst-forming unit erythroid (BFU-E) colonies were observed. Approximately 4.5% of culture cells are CFU-E. ND: not detectable.