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. 2013 Oct 15;22(2):451–463. doi: 10.1038/mt.2013.201

Figure 2.

Figure 2

Extensive ex vivo expansion of human erythroblasts after ectopic expression of genetic factors in cord blood (CB)-derived primary erythroblasts. (a) Growth curves of human erythroblasts after retrovirus transduction with different combinations of genetic factors in day 19 CB primary erythroblasts. O: Oct4, S: Sox2, K: Klf4, M: c-Myc, P: p53shRNA, and green fluorescent protein (GFP). (b) Independent experiment showing growth curves of human erythroblasts after retrovirus transduction with different combinations of four and three genetic factors to further narrow down essential factors. (c) FACS analysis of cell surface markers and intracellular hemoglobin protein expression of iE2 cells at day 50, 84, 110, 127, and 185 of expansion culture. (d) Cell morphology in suspension culture well (left) or cytospin by Wright-Giemsa and benzidine staining (middle and right) of iE2 cells at days 132 and 295 of expansion culture. Almost all of the cells have proerythroblasts or basophilic-erythroblasts morphology and are benzidine negative suggesting low hemoglobin content. Scale bar: 10 µm.