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. 2013 Dec 24;22(2):303–311. doi: 10.1038/mt.2013.212

Figure 2.

Figure 2

Expression of TALENs in cells. (a) Huh7 cells transfected with the plasmids encoding indicated Flag-tagged TALENs were lysed 48 hours post-transfection and the lysates were analyzed by immunoblotting with monoclonal antibody against Flag. (b) Huh7 cells were co-transfected with the plasmids encoding TALENs along with pEGFP. Immunofluorescence assay was performed 48 hours post-transfection. (c) The viability of the control cells and the cells expressing TALENs was measured by CCK8 assay every day for 4 days. TALEN, transcription activator-like effector nucleases.