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. 2013 Dec 24;22(2):303–311. doi: 10.1038/mt.2013.212

Figure 3.

Figure 3

The effect of TALENs on the production of viral proteins and pgRNA. (a–d) Huh7 cells were co-transfected with the linear full-length HBV DNA (genotype A) and the plasmids encoding indicated TALENs. Forty-eight hours post-transfection, the supernatants were collected to detect HBeAg (a) and HBsAg (b) by ELISA and the cells were harvested for western blotting to detect the HBcAg expression (c) or for RT real-time PCR analysis to identify the level of pgRNA (d). ELISA, enzyme-linked immunosorbent assay; HBV, hepatitis B virus; TALEN, transcription activator-like effector nucleases.