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. 2013 Nov 26;22(2):278–291. doi: 10.1038/mt.2013.231

Figure 7.

Figure 7

scL-mediated in vivo targeting of cancer stem-like cells (CSCs) in chemically induced mouse tumors. (a) Ex vivo fluorescence imaging of chemically induced lung tumors in A/J mice 24 hours after i.v. injection with 100 µg of 6FAM-ODN. Green fluorescence representing uptake of 6FAM-ODN in lung metastatic tumors was quantitatively determined as in Figure 4. (b) 6FAM-ODN uptake in lung CSCs (CD34+c-Kit+Sca-1+CD45−) was determined by flow-activated cell sorting (FACS) analysis and compared in graph c. (d) Ex vivo fluorescence imaging and (e) FACS analysis of liver tumors in A/J mice i.v. treated with scL-6FAM-ODN for 24 hours. Arrows indicate chemically induced liver tumors. (f) 6FAM-ODN uptake in mouse hematopoietic stem cells (mHSCs, CD34−c-Kit+Sca-1+CD45+Lin−) and mouse mesenchymal stem cells (mMSCs, CD34−c-Kit−Sca-1+CD45−Lin−) were monitored in the bone marrow and blood by FACS. Statistical significance was determined using analysis of variance (*P < 0.05; **P < 0.001).