Figure 1.

Selection of siRNA-E sequence for insertion into the AAV9 vector. (a) Small interfering RNA sequences targeting TRPV1. (b) Short-hairpin RNA (shRNA) sequences targeting TRPV1. (c) Schematic representation of the plasmid vector used in vitro. This plasmid encodes firefly luciferase and a fusion renilla luciferase and TRPV1. (d) Relative TRPV1 expression level in vitro as determined by renilla/firefly ratio. Data are presented as means ± SEM. (e) The AAV9-shTRPV1 vector, including the shRNA sequence driven by the human polymerase III human U6 promoter and hGH inserted downstream of the shRNA sequence for the AAV9 genome detection assay, is shown. AAV, adenoassociated virus; hGH, human growth hormone; HSV-TK, herpes simplex virus-thymidine kinase; ITR, inverted terminal repeat; SC, scramble; SV, Simian virus; TRPV1, transient receptor potential vanilloid 1.