Figure 5.

The reverse of aberrant alternative splicing by artificial site-specific RNA endonucleases (ASREs) expression in myotonic dystrophy type 1 (DM1) cells. Semi-quantitative reverse transcriptase–polymerase chain reaction was used to detect different alternative splicing isoforms for (a) insulin receptor (a), (b) bridging integrator-1, (c) cTNT, (d) Nfix, (e) SMYD 1, and (f) SERCA1 in differentiated DM1 cells (GM04602). We used differentiated normal human fibroblast and DM1 cells transfected with vector only as controls. At least two independent experiments were carried out, with the means and SD of the percent-splice-in (psi) shown below a representative gel. In all panels, the P values were calculated by t-test comparing with vector only (only significant difference was shown).