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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: FEBS J. 2013 Dec 24;281(3):957–969. doi: 10.1111/febs.12661

Figure 2. Hyperoxia increased g-actin and f-actin content.

Figure 2

Representative western blots of g-actin and f-actin from the same well for each condition, and GAPDH as a loading control for g-actin (upper panel). Equivalent volumes from the Triton-soluble and Triton-insoluble fractions were loaded onto the same gel for comparison. The lower panel shows the densitometry for g-actin normalized to GAPDH and the densitometry for f-actin. Both g- and f-actin were further normalized to the normoxia condition. * indicates a significant difference, p<0.05; N.S. indicates not significant; n=4, error bars indicate standard error.