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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: FEBS J. 2013 Dec 24;281(3):957–969. doi: 10.1111/febs.12661

Figure 3. Hyperoxia increased nuclear size, and ROCK inhibition did not prevent the increase.

Figure 3

Panel A: Fluorescence images of MLE-12 cells stained with Hoechst33342. Compared to normoxia (A), hyperoxia (B) caused nuclear swelling. Y-27632 did not influence nuclear size (C, D). Scale bars are 50μm. Panel E: Quantification of nuclear size in normoxia and hyperoxia treatment with and without Y-27632. Nuclei (>120) from 3-5 fields in 3 different experiments were measured. Panel F: Quantification of nuclear height in normoxia and hyperoxia treatment with and without Y-27632. Nuclei (~100) from 5 fields in 3-4 different experiments were measured. * indicates significant differences from normoxia treatment; p<0.05; error bars indicate standard error.