Cells were treated for 48 h with normoxia (A and B) or hyperoxia (C, D, E, and F) and then kept static (A, C, and E) or exposed to 20% cyclic stretch, 15 cycles/min (B, D, and F) for 60 min. Cells in E and F were treated with Y-27632 during the hyperoxia treatment. For Panels A-F areas of cell detachment were identified by the user, filled in automatically by Photoshop (indicated by dark areas), and quantified using Matlab as described in Methods. Panel G: Quantification of cell detachment: percentage of area of detached cells was determined for at least 5 fields from at least 3 different wells (n=3); three-way ANOVA was performed with the Holm-Sidak method used for comparisons; * indicates a significant difference from stretched, normoxia treated cells; ** indicates a significant difference from stretched, hyperoxia treated cells; p<0.05; standard errors are indicated.