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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: FEBS J. 2013 Dec 24;281(3):957–969. doi: 10.1111/febs.12661

Figure 6. Hyperoxia caused an increase in focal adhesion area but did not increase total vinculin.

Figure 6

Panel A: Representative western blots of vinculin and GAPDH and densitometry summarized from 3 experiments. Panels B-E: Immunofluorescence images of focal adhesions in MLE-12 cells stained for vinculin. Compared to normoxia (B), hyperoxia for 48h (C) caused a thickening of the focal adhesions. Treatment with the Rho Kinase inhibitor (Y-27632, D and E) reduced the localization of vinculin in both normoxia (D) and hyperoxia-treated (E) cells. Scale bars are 10μm (representative images from 3 different experiments). Panel F: Quantification of focal adhesion area in normoxia and hyperoxia-treated cells. Focal adhesions (>1500) from 5-7 fields for each condition in 3 different experiments were measured.