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The co-transgenesis assay and GFP expression in the resulting embryo detected by in situ hybridization. (A) A schematic representation of the co-injection of a CNE fragment, the reporter cassette and the sperm nucleus into Xenopus eggs. (B, C) GFP expression in the embryo co-injected with the C element of Xenopus Pax6 and the GFP reporter cassette. Arrows indicate eye-specific expression of GFP (B, lateral view; C, frontal view).
