Table 1.

The reaction mixture and cycle setting used for amplification of the C region of Xenopus Pax6

Reagents	Volume (μL)
Distilled water	57
10 × Pfx buffer	20
50 mM MgSO4	2
dNTP mix (2.5 mM each)	12
C-1 Primer (10 pmol/μL)	3
C-2 Primer (10 pmol/μL)	3
X. tropicalis genomic DNA (50 ng/μL)	2
Platinum Pfx DNA polymerase (2.5 U/μL)	1

The cycle setting: 94°C 5 min. → (94°C 30 sec. → 58°C 1 min. → 68°C 1 min.) × 38 cycles → 68°C 7 min.