Figure 4. Oxidative stress response pathway mutants do not have an increased mtDNA mutation frequency.

(A) Lifespan of w¹¹¹⁸ and Sod2 mutant flies. The median lifespan (indicated by dashed lines) was 60 days for w¹¹¹⁸ flies and 41 days for Sod2 mutants. (B) The mutation frequency using pooled data from all three TaqI sites in old Sod2 mutants (39- to 41-day-old), young Ogg1^ins/Ogg1^ins; Sod2 double mutants (1- to 3- day-old), old Ogg1^ins/Ogg1^ins; Sod2 double mutants (26- to 28- day-old), and age-matched w¹¹¹⁸ controls. The 26- to 28- day-old control estimate was obtained from linear regression of the age-dependent w¹¹¹⁸ mutation frequency. Horizontal bars represent the median mutation frequency, and error bars indicate the interquartile range. There was no significant difference in mutation frequency between mutants and age-matched controls (Mann-Whitney unpaired U tests).