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. 2014 Feb 6;10(2):e1003471. doi: 10.1371/journal.pcbi.1003471

Figure 6. Hsp72 overexpression differentially regulates IκBα transcription.

Figure 6

A. Model simulations with the nominal parameters predict that A20 and IκBα mRNA are similarly induced (blue solid lines). The model with modified parameters (kiA20 set to 10-fold the nominal value, kc2a 1/6 the nominal value, and initial p65 70% of the nominal value) was simulated to find the transcript levels assuming that Hsp72 regulates the network by reducing total p65 and by inhibiting both upstream IKK activation and IκBα phosphorylation (red dashed lines). mRNA levels for IκBα and A20 transcripts are normalized with respect to their initial levels. B. IκBα and A20 mRNA transcript levels (fold change) in all three cell types were measured using qPCR at the indicated times following TNFα addition. IκBα and A20 transcripts are expressed similarly in BV2 and LacZ cells, while relative A20 is decreased in Hsp72 cells. Mean levels in Hsp72 cells are significantly different (P<0.05) from both BV2 and LacZ cells determined by ANOVA and Newman-Keuls post hoc tests at 30, 60 and 120 min (n = 3).