Table 2. Modified model parameters to simulate possible regulation of steady state protein levels in the NF-κB signaling network.

Change to model	Biological interpretation	Simulated results
Decreased c2a (Equation 7)	Inhibition of induced IκBα protein due to heat shock proteins [39]	Figure 4A
Decreased [IkBaNFkB(0)] (Equation 11)	Reduction of steady state p65 protein levels due to Hsp72 overexpression (unknown mechanism)	Figure 5A
Decreased [IKKn(0)] (Equation 2)	Reduction of neutral IKK protein levels due to Hsp72 interactions with NEMO preventing proper IKK complex formation [15]	Figure S5A

Hsp72 interactions with the NF-κB signaling network are interpreted as constant perturbations to rates or initial species corresponding to the relevant biological reaction. Equation numbers reference the complete model equations shown in Figure 2, with altered parameters colored in red (inhibition) or blue (enhancement).