A. PCR of Leishmania internal transcribed spacer 1 (ITS1) region amplified within pools of female Sergentomyia sand flies captured indoors and Leishmania spp. controls. M: 100 bp size marker; Lanes 1 and 2 (L. major and L. tropica reference strains, respectively); Lanes 3 to 6, S. ingrami pools, Lane 7, S. hamoni pool; Lane 8, S. africana africana pool (∼500 bp) and Lane 9, negative control. B.
HaeIII digestion of restriction fragment length polymorphisms of ITS1 PCR products shown in A. M: 100 bp size marker; Lane 1, L. major (IPAP/EG/1989/S1-177); Lane 2, L. tropica (MHOM/SU/1974/K27); Lanes 3 to 6, S. ingrami pools; Lane 7, S. hamoni pool and Lane 8, S. africana africana pool.