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. 2014 Feb 6;8(2):e2630. doi: 10.1371/journal.pntd.0002630

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© 2014 Nzelu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. PCR of Leishmania internal transcribed spacer 1 (ITS1) region amplified within pools of female Sergentomyia sand flies captured indoors and Leishmania spp. controls. M: 100 bp size marker; Lanes 1 and 2 (L. major and L. tropica reference strains, respectively); Lanes 3 to 6, S. ingrami pools, Lane 7, S. hamoni pool; Lane 8, S. africana africana pool (∼500 bp) and Lane 9, negative control. B. HaeIII digestion of restriction fragment length polymorphisms of ITS1 PCR products shown in A. M: 100 bp size marker; Lane 1, L. major (IPAP/EG/1989/S1-177); Lane 2, L. tropica (MHOM/SU/1974/K27); Lanes 3 to 6, S. ingrami pools; Lane 7, S. hamoni pool and Lane 8, S. africana africana pool.